Optimized protocol for DNA extraction from ancient skeletal human remains using Chelex-100

Extraction and Analysis of Ancient DNA from Human Remains of Masjede Kabood Burial Site

Authenticating DNA Extracted From Ancient Skeletal Remains

The survival of DNA, the most informative biological molecule, for periods of at least several thousand years in bone was demonstrated more than four years ago. However, difficulties with authenticating ancient DNA have made diagenetic studies problematic. It is therefore essential that these problems be overcome before the question of DNA survival can be addressed. Here we describe our work wi...

A Simple Chelex Protocol for DNA Extraction from Anopheles spp.

Endemic countries are increasingly adopting molecular tools for efficient typing, identification and surveillance against malaria parasites and vector mosquitoes, as an integral part of their control programs. For sustainable establishment of these accurate approaches in operations research to strengthen malaria control and elimination efforts, simple and affordable methods, with parsimonious r...

Comparison of two ancient DNA extraction protocols for skeletal remains from tropical environments.

OBJECTIVES The tropics harbor a large part of the world's biodiversity and have a long history of human habitation. However, paleogenomics research in these climates has been constrained so far by poor ancient DNA yields. Here we compare the performance of two DNA extraction methods on ancient samples of teeth and petrous portions excavated from tropical and semi-tropical sites in Tanzania, Mex...

DNA preservation: a microsatellite-DNA study on ancient skeletal remains.

To determine the effect of environmental factors on the preservation of DNA, archeological teeth of approximately similar age but greatly differing site milieu were examined for DNA content. The complex relational system of locational milieu of the samples was reduced to its essential and, at the same time, easily measurable factors. These are temperature, humidity, pH value, the geochemical pr...